What is multiplex PCR? - Front Line Genomics
Jul 26, 2021 · Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – the largest known human gene. The dystrophin complex connects the cytoskeleton of muscle cells ...
Sample Preparation for NGS – A Comprehensive Guide
Aug 21, 2024 · Step #3: Amplification. This is an optional step, but it is usually required. It is dependent on the application of NGS and the sample size. Amplification becomes essential to obtain enough coverage for reliable sequencing for samples with small amounts of …
A brief history of Next Generation Sequencing (NGS)
Jul 26, 2021 · 2000: Lynx Therapeutics Company launched the first of the NGS technologies, called Massively parallel signature sequencing (MPSS). The company was later bought by Illumina. 2004: 454 Life Sciences marketed a new generation pyrosequencing technology, called the Roche GS20. It was the first NGS platform on the market.
Homepage - Front Line Genomics
Registered Office: Ground Floor, Cromwell House, 15 Andover Road, Winchester, Hampshire, SO23 7BT, United Kingdom. Tel: +44 (0)208 191 8810 Email: info ...
The evolution of DNA sequencing technologies
Feb 12, 2021 · At the time, this technique was revolutionary. It enabled sequencing of 500-1,000bp fragments. A variant of the Sanger method – plus and minus method – developed by Sanger and Alan Coulson led to the first sequence of a DNA genome, that of bacteriophage φX174, in 1977. Two years later Allan Maxam and Walter Gilbert published their chemical ...
The Sequencing Buyer’s Guide: 5 th Edition - Front Line Genomics
The Sequencing Buyer’s Guide: 5th Edition. The Sequencing Buyer’s Guide: 5. Edition. In recent years, next-generation sequencing (NGS) has evolved from a technology used purely in research settings to being a key feature of the clinical diagnostics pipeline – fully automated sequencing is now available at a lower cost per base and with ...
How-to: perform a genome-wide association study (GWAS)
Sep 10, 2024 · Genome-wide association studies are commonly used in the genomics world to identify variants associated with a trait or disease.During a GWAS, researchers will identify areas of the genome that show a strong signal linked to the trait in question; that is, in the discovery cohort, areas or specific markers in which variation is more common than in the controls.
Long-read sequencing vs short-read sequencing
Jul 26, 2021 · Long-read sequencing. Long-read sequencing technologies are capable of reading longer lengths, between 5,000 and 30,000 base pairs. Therefore, they immediately address one of the main challenges faced by short-read sequencing. They sequence a single molecule, eliminating amplification bias, and generate a reasonable length to overlap a sequence ...
Evolution of cloning: A Dolly good show! - Front Line Genomics
Sep 14, 2021 · Evolution of cloning: A Dolly good show! Shannon Gunn 14 September 2021. My first real memories of cloning came from a film by one of my favourite childhood bands S Club 7. In the film, Seeing Double, the band – as you can guess – are cloned by an ‘evil’ scientist (the classic scenario) who aims to take over the world with his army of ...
A longstanding mystery: How do identical twins arise?
Oct 1, 2021 · Scientists have discovered DNA methylation differences that are unique to identical twins, helping to unravel the exact molecular events that lead to division of a zygote.. Monozygotic twins, or identical twins, result from the fertilisation of a single egg with a single sperm.At some point during the very early pre-implantation stages of …